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KMID : 1161520090130030297
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Animal Cells and Systems
2009 Volume.13 No. 3 p.297 ~ p.304
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Gene expression and regulation of wax moth transferrin by PAMPs and heavy metals
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Han Jik-Hyon
Lee Ji-Sook
Lee Chang-Seok
Koh Sang-Kyun
Seo Sook-Jae
Yun Chi-Young
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Abstract
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A complete mRNA sequence of transferrin from the wax moth, Galleria mellonella, was obtained, and compared with those of other species. We previously reported that the sequence was most similar to those of Manduca sexta and Bombyx mori. As in other moths, G. mellonella transferrin had only one iron?binding site at its N?terminal region. Semi?qRT PCR was conducted to investigate tissue?specific distribution and transcriptional regulation of the wax moth transferrin mRNA. Larval muscle and fat body contained larger quantity of mRNA than other tested tissues. In this study, it was observed that iron and cadmium regulated transferrin transcription, and this regulation pattern was tissue specific. Iron up?regulated transferrin mRNA level in fat body, while suppressed it in the Malpighian tubules and silk glands. Cadmium decreased the mRNA level in fat body, muscle, and Malpighian tubules, but significantly increased the mRNA level in silk glands. In addition, the mRNA expression was induced by all tested pathogen?associated molecular patterns (PAMPs) including LPS, lipoteichoic acid (LTA), glucan, and even chitin.
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KEYWORD
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transferrin, wax moth, cDNA cloning, pathogenassociated molecular pattern, PAMP, iron, cadmium
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